DNA extraction lab - 1823 Words

DNA Extraction of a Musa acuminata Biology Lab Report \Analyze and Interpret A number of steps are required to isolate DNA from cellular contents. Describe what happens at each step, and why it acts to separate the parts of the cell. /6 There are three specific steps required to isolate DNA from its cellular contents. The steps used to remove and expose DNA from its cell are: breaking down the food type you are using by crushing it, for example a banana or strawberries, exposing the substance to a sodium chloride (NaCl) solution, subjecting the product to detergent solution (dH2O), filtering the solution and lastly, the addition of ethanol. When beginning with a solid substance, such as a banana, crushing the substance allows for†¦show more content†¦Firstly, DNA is constantly trying to coil itself back into its original position, which is a supercoiled polymer. So when the DNA is exposed to a stirring rod in a circular motion the DNA tries to coil itself to the rod. Secondly, another property of DNA, which is the fact that it is polar, causes it to attract to the polar glass rod. These two properties working together allow the DNA to coil around the stirring rod. Conclude and Communicate Based on your procedure and results, how is DNA isolated from plant or animal cells? /4 When looking at DNA extraction, the key steps for isolating DNA from a plant cell or animal cell is to pass through the protective barriers of each cell – whether it is a cell wall and cell membrane or only the cell membrane for an animal cell. In order to extract DNA from a banana, the first step required is to place half of it in a small resealable plastic bag without air bubbles, and then using your fingers mash the banana until no visible chunks. Next, using a graduated cylinder measure 10 mL of the 0.1% NaCl solution, and add it to the bag – mix thoroughly. Then the next step is with a graduated cylinder measure 3 mL of detergent solution (dH2O), add it to the bag and mix gently, so as not to form bubbles. After this, set up a retort stand with the gas tube using a retort clamp, strain the banana mixture through a filter paper, and collect the filtrate in a gas tube for approximatelyShow MoreRelatedDna Extraction Lab Report1627 Words   |  7 PagesTan 1 DNA EXTRACTION Aim : To extract the DNA from an egg yolk using various enzymes and to compare with other groups the most effective way to extract DNA. Hypothesis : To be able to observe white springy substances after mixing with enzyme and alcohol. Apparatus : -Test tube, spatula, glass rod, dropper, beaker, test tube rack, skewer. Materials : - 1 egg, meat tenderizer, salt, water , soap, isopropyl alcohol 91%, pineapple juice. Variables : Manipulated Variable : RespondingRead MoreLab Experiment : Diffusion And Osmosis1565 Words   |  7 PagesLab 6: Diffusion and osmosis Honey I Shrunk the Carrot is the tittle of Lab 6. In this lab, the objective was to observe the changes in size of a carrot. The tip of both carrots was removed and a string was tied around both carrots. Two Styrofoam cups of distilled water was prepared and two grams of salt was added to one cup. One carrot was placed in the salt water, and another was placed in the distilled water. Both cups were put to the side, and sat for twenty-four hours. At the end of the twenty-fourRead MoreDNA EXTRACTION Essay1195 Words   |  5 Pageslearned from studying an organisms DNA. The first step to doing this is extracting DNA from cells. In this experiment, you will isolate DNA from the cells of fruit. Materials (1) 10 mL Graduated Cylinder(2) 100 mL Beakers15 cm Cheesecloth1 Resealable Bag1 Rubber Band (Large. Contains latex pleasewear gloves when handling if you have a latex allergy).Standing Test TubeWooden Stir StickFresh, Soft Fruit (e.g., Grapes, Strawberries, Banana, etc.) ScissorsDNA Ex traction SolutionIce Cold EthanolYou MustRead MoreLab 13 Essay1011 Words   |  5 PagesLester Biology Lab 13 1. Arrange the following molecules from least to most specific with respect to the original nucleotide sequence: RNA, DNA, Amino Acid, Protein Protein, amino acid, RNA, DNA 2. Identify two structural differences between DNA and RNA. Two structural differences between DNA and RNA would be that RNA contains ribose while DNA is missing the hydroxyl group form its ribose. Also, RNA is created by transcription of a gene locate in the cells of DNA. DNA is doubled strandedRead MoreThe Community of Microorganisms that Reside in the Epithelia of Humans1034 Words   |  5 Pagesor nose. This lab consisted of the collection of skin bacterial organisms and amplification of the 16s rRNA to construct a small molecular phylogeny of the human body microbiome, or the community of microorganisms that reside in the epithelia of humans. This information could only be acquired through processes such as DNA extraction, amplification of specific genetic target by the polymerase chain reaction (PCR), agarose gel electrophoresis, restriction enzyme digestion, cloning of DNA fragments intoRead MoreThe Forensic Analysis Of Dna972 Words   |  4 PagesEmulation of the Forensic Analysis of DNA Introduction All living beings possess DNA that is unique to them.2 For this reason DNA is often used in criminal investigations to help ascertain a perpetrators identity1. In this lab we intend to mimic the methodology involved in such a process. DNA consists of thymine, guanine, cytosine and adenine in a double helix structure, in a sequence that is unique to each person.2 A lysis solution is to be used to safely isolate onion DNA from tissue, consisting of sodiumRead MoreDna Analysis : Strawberry Dna Extraction1685 Words   |  7 Pages Strawberry DNA Extraction Sara Awad 795149 Read MoreGenetic Engineering Of People : How Dna Is Located, Extracted And Amplified1055 Words   |  5 Pages Genetic Engineering of People: How DNA is Located, Extracted and Amplified Eric Ottoson Chemeketa Community College Abstract Genetic engineering is controversial topic of discussion amongst many circles today. The benefits can be laid out to its untold potential, but there are lines that are superimposed from moral standpoints and personal ethics. The idea resonating in these circles brings to light the availability to be able to selectively choose your offspring and specific featuresRead More95% Ethanol (Ethyl Alcohol Denatured) . Shampoo Pantene.983 Words   |  4 Pages pH paper DNA stands for deoxyribonucleic acid. DNA is the set of nongenetic traits, qualities, or features that characterize a person or other living thing. Extracting DNA helps understand different types of genes, as there are 500 different types of genes in total and counting. DNA can be found everywhere. In every cell, in every tissue, in every organ and as a matter a fact in every living thing. DNA is the only known molecule that is able to reproduce through replication. DNA is made in theRead MoreGenetics Module 7 Lab 2 Essay1692 Words   |  7 Pagesï » ¿******************************************************************************************** Answer Sheet—Module 7 Lab DNA Extraction Click on the following link and view the DNA extraction: http://learn.genetics.utah.edu/content/labs/extraction/ 1. What is the source of the cells used in this demonstration? A human. 2. Give three practical uses of DNA that is extracted:   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   a. Genetic testing   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   b. Body Identification   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   c. Forensic analysis 3. Name the piece of